Fig. 2

CHML overexpression promotes HCC cell metastasis and invasion. a Dot plots comparing CHML expression in patients with or without microvascular invasion (MVI) in two published datasets. *P < 0.01, Student’s t-test. b Dot plot analysis of CHML expression in normal, HCC and portal vein tumor thrombus (PVTT) tissues. N normal, T tumor, P PVTT. **P < 0.01 by two-tailed unpaired Student’s t-test. c Western blot analysis of CHML expression in normal, HCC, and PVTT tissues obtained from 4 patients. d Western blot analysis of CHML expression in 5 HCC cell lines. Arrow indicated the CHML band. e Western blot analysis of the overexpression efficiency of CHML in HCC cell line PLC/PRF/5 and YY8103. v vector control. f Boyden chamber and invasion assay are conducted to detect the migratory and invasive ability of control cells and CHML-overexpressed cells. Each representative image is shown. g Quantitative results are respectively illustrated for panel (b). Data are shown as the mean ± s.e.m., **P < 0.01 by two-tailed unpaired Student’s t-test. All data are represented as mean ± s.e.m.