Fig. 1
From: Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution
The SPA cryo-EM of SA. a A representative micrograph of the SA specimen by the VPP-Cs-corrector-coupled cryo-EM. The scale bar represents 20 nm. b Representative 2D class averages of SA particle images. The scale bar represents 5 nm. c The 3D reconstruction of apo-SA at 3.3 Å resolution from 23,991 particles and d the 3D reconstruction of the biotin-bound SA at 3.2 Å resolution from 45,686 particles. e The fourier shell correlation (FSC) curves of the two reconstructions using the gold-standard criteria
