Fig. 4

Reconstruction and classification using the mixed dataset. a The 3D reconstruction of the mixed dataset (apo-SA + biotin-SA) with 3.1 Å resolution demonstrates a biotin-bound-like density (circled in blue) as b the 3D reconstruction of biotin-SA at 3.2 Å resolution of a monomer. c Asymmetric 3D classification reconstructions of the mixed dataset. The empty density of the biotin-binding pocket in the monomer of the Class II reconstruction is circled in red in contrast to the ligand densities in the other classes. The percentage of particles and ligand occupancy in each class are labeled. A column graph with error bars to show the ligand occupancy of each class is shown. The 3D reconstruction of d class II and e the merged class I–III–IV indicated the apo-SA and biotin-SA individually. A side section comparison demonstrated the extra density of loop 46–51 and the biotin molecule in e (blue circle). Error bars (SD) were calculated from three random repeats. Source data are provided as a Source Data file