Fig. 3
From: Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents
Cordycepin prevents radiation ulcer. a Images of hind limb from irradiated rats (control, prevention, treatment) on day 0, 15, and 35. This test was repeated three times. Representative images were shown. Control: Rat right posterior limb (other parts covered with lead board) was exposed to 40 Gy radiation under anesthesia; Prevention: Cordycepin were injected before radiation; Treatment: Cordycepin were injected after radiation. b Histological analysis of the skins from normal rats and irradiated rats (control, prevention, treatment) 35 days after radiation. c Histological analysis of intestinal sections from normal mice and irradiated mice (control, prevention, treatment) 4 days after radiation. This test was repeated three times. Representative images were shown. Control: Mouse abdominal (other parts covered with lead board) was exposed to 12 Gy radiation under anesthesia. Prevention: Cordycepin were injected before radiation; Treatment: Cordycepin were injected after radiation. d Quantitation of surviving crypts (n = 3) and villus height (n = 6) from normal mice and irradiated mice (control, prevention, treatment) 4 days after radiation, the villus height was calculated using Image J software. e Survival curve of mice subjected to abdominal radiation (n = 23). f Representative pictures of tongues stained with toluidine blue from normal mice and irradiated mice (control, prevention, treatment) 10 days after radiation. Lack of protective epithelial barrier and therefore ulcer formation is indicated by deep, royal blue staining in epithelium defects. This test was repeated three times. Representative images were shown. Control: Mouse head and neck area (other parts covered with lead board) was exposed to 15 Gy radiation under anesthesia. Prevention: Cordycepin were injected before radiation; Treatment: Cordycepin were injected after radiation. g Histological analysis of the tongues from normal mice and irradiated mice (control, prevention, treatment) 10 days after radiation. Bars represent 500 μm (b), 500 μm(top) and 100 μm(bottom) (c), 300 μm (f), 100 μm (g). Data in d and e represent the means ± S.D. (**P < 0.01; student’s t-test)
