Fig. 6
From: Efficient base editing for multiple genes and loci in pigs using base editors
Immunocytes and V(D)J rearrangement analysis of RAG1, RAG2, and IL2RG mutant piglets. a qRT-PCR analysis of IL2RG, CD4, CD8, and CD19 expression in peripheral blood of RAG1, RAG2, and IL2RG mutant piglets. b–d FACS analysis of mature B (b), T (c), NK (d) cells from the peripheral blood. Cells were stained with antibodies of anti-CD3, IgM CD8 to detect mature B (b), T (c), and NK (d) cells. e, f TCR-β gene rearrangement analysis in the peripheral blood and thymus. g, h, and i IgH gene rearrangement analysis in the peripheral blood (g), spleen (h), and bone marrow (i). Source data are provided as a Source Data file
