Fig. 1

Germinal centre B cells are reduced in the Peyer’s patches of aged BALB/c and C57BL/6 mice. Flow cytometric analysis of germinal centre (GC) cell populations in the Peyer’s patches (PPs) of adult (3-month-old) and aged (22-month-old) BALB/c and C57BL/6 mice. a–c Representative flow cytometric plots (a) and quantitation of B220⁺Ki67⁺Bcl6⁺ GC B cells (b, c) in the PPs of 3-month-old and 22-month-old BALB/c mice. d, e Quantitation of B220⁺Ki67⁺Bcl6⁺ GC B cell percentage (d) and number (e) in C57BL/6 mice. f–h Representative flow plots (f) and quantitation of CD4⁺Foxp3⁻CXCR5⁺PD-1⁺ Tfh cells (g, h) in the PPs of BALB/c mice. i, j Quantitation of Tfh cell percentages (i) and numbers (j) in C57BL/6 mice. k–m Representative flow plots (k) and quantitation of CD4⁺Foxp3⁺CXCR5⁺PD-1⁺ Tfr cells (l, m) in BALB/c mice. n, o Quantitation of Tfr cell percentages (n) and numbers (o) in C57BL/6 mice. Bar graphs show the combined results of 3–6 independent experiments which were performed with female BALB/c mice and both male and female C57BL/6 mice with a total of n = 11–49 mice per group. Bar height corresponds to the mean, and each circle represents one biological replicate. P-values were determined using the Mann-Whitney test in GraphPad Prism6. Source data are provided as a Source Data file