Fig. 7

Faecal microbiota transplantation between adult mice of different strains does not affect gut germinal centres. a, b 16S rRNA sequencing data generated from faecal pellets collected from adult 3-month-old C57BL/6 and BALB/c mice. (a) Bray-Curtis PCoA comparing samples from 3-month-old BALB/c and C57BL/6 mice. b Shannon diversities, with a p-value based on ANOVA tests. c–h Three-month-old C57BL/6 mice were given a suspension of faecal pellets taken from 3-month-old BALB/c mice by oral gavage to achieve faecal microbiota transplantation (FMT). In addition, the cages of C57BL/6 recipients were supplemented with fresh faecal pellets and dirty bedding from BALB/c mice once a week. Control mice were gavaged with PBS. The percentage and number of B220⁺Ki67⁺Bcl6⁺ GC B cells (c, d), CD4⁺Foxp3^-CXCR5⁺PD-1⁺ Tfh cells (e, f) and CD4⁺Foxp3⁺CXCR5⁺PD-1⁺ Tfr cells (g, h) in Peyer’s patches as quantified by flow cytometry. Bar plots show the combined results of two experiments with a total of n = 14–15 mice per group. Bar height corresponds to the mean, each circle represents one biological replicate. P-values were determined using the Mann-Whitney test in GraphPad Prism6. Source data are provided as a Source Data file