Table 1 List of antibodies used for flow cytometry
From: Heterochronic faecal transplantation boosts gut germinal centres in aged mice
Antibody | Supplier (Clone) | Dilution |
|---|---|---|
PE/PE-Cy7-coupled anti-mouse Bcl6 | BD Biosciences (K112-91) | 1:100 |
APC-AF780-coupled anti-mouse PD1 | eBioscience (J43) | 1:200 |
APC/Foxp3-coupled anti-mouse Foxp3 | eBioscience (FJK-16S) | 1:100–1:200 |
AF488/AF700-coupled anti-mouse Ki67 | eBioscience (SolA15) | 1:100 |
BV421-coupled anti-mouse CXCR5 | Biolegend (L138D7) | 1:100 |
V500/PE/BV605-coupled anti-mouse CD4 | Biolegend (RM4-5) | 1:400–1:800 |
BV510/BV785-coupled anti-mouse B220 | Biolegend (RA3-6B2) | 1:200–1:400 |
PerCp-Cy5.5-coupled anti-mouse CD44 | Biolegend (IM7) | 1:200 |
AF674/FITC-coupled anti-mouse IgA | Southern Biotech (1040-02/-31) | 1:100 |
APC-Cy-7-coupled anti-mouse IgK | BD Biosciences (187.1) | 1:100 |
