Fig. 8 | Nature Communications

Fig. 8

From: IAPP toxicity activates HIF1α/PFKFB3 signaling delaying β-cell loss at the expense of β-cell function

Fig. 8

PFKFB3 silencing restores Ca2+ dynamics of cytosolic, ER and mitochondrial compartment. Average traces ± SEM of a cytosolic, b endoplasmic reticulum (ER), and c mitochondrial calcium in INS 832/13 cells overexpressing LacZ (LacZ_CTRL siRNA) or hIAPP in the presence or absence of PFKFB3 siRNA (hIAPP_PFKFB3 siRNA and hIAPP_CTRL siRNA, respectively) measured at low (2.8 mM, 2.8 G) and high (16.8 mM, 16.8 G) glucose. Cyclopiazonic acid (CPA) and sodium azide (NaN3) have been added after measurement of [Ca2+] in high glucose (16.8 mM) to establish the [Ca2+] content in ER and mitochondria, respectively. Average Ca2+ traces are presented from one of 3 (n = 3) independent experiments. The vertical axis in LacZ_CTRL siRNA for D4ER 535/470 ratio has been shown within 0.99–1.015 AU to indicate the increment in average traces upon exposure to high (16.8 mM) glucose. Ca2+ traces shown for ER and mitochondria were first normalized to their respective nadirs, obtained in the presence of CPA or NaN3

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