Fig. 2
From: LUX ARRHYTHMO mediates crosstalk between the circadian clock and defense in Arabidopsis
LUX regulates stomata aperture and defense signaling. a Light and time scheme used for some treatments. 25-day-old plants grown in LD were transferred to LL for 1 day followed by pathogen infection 25 or 37 h after onset of LL. The light intensity was lowered from 180 to 10 µmol m−2 s−1 photon flux density at 49 h LL or 61 h LL for plants infected at 25 h LL or 37 h LL, respectively. b Bacterial growth (left) and photographs (right) of plants sprayed with PmaDG3 (OD600 = 0.1) at LL25 or LL37 (n = 6). c Bacterial growth of plants infiltrated with PmaDG3 (OD600 = 0.0001) at LL25 (white bars) or LL37 (black bars) (n = 6). d Stomatal aperture measured at 1 hpi of PmaDG3 (OD600 = 0.1) at LL25 or LL37 (n = 80). e Systemic acquired resistance (SAR) assay (n = 6). SAR was induced by the primary infection of Pma avrRpt2 (OD = 0.05). Bacterial growth (left) and disease symptom (right) was recorded with the secondary infection of PmaDG3 (OD = 0.0001) at 3 dpi in LD. f Seedling growth inhibition assay with 1 µM flg22. The fold difference was calculated as the ratio of water-treated root length/flg22-treated root length of each genotype. Data (left) represent the average of three independent experiments (n = 4 per genotype/treatment in each experiment). Pictures of seedlings (right) were from one representative experiment. g Quantification (left) and images (right) of callose deposition with flg22 (1 µM) or Pma hrcC− (OD600 = 0.1) treatment (n = 6). Numbers of callose deposits were quantified using ImageJ (version 1.45). H2O treatment did not induce callose deposition (not shown). Data represent mean ± SEM. Except f, other experiments were repeated two times and similar results were obtained. Statistical analysis was performed with one-way analysis of variance with post hoc Tukey honestly significant difference test. Significant difference between Col-0 and other plants at the same time point and/or with the same treatment was indicated by different letters or by asterisks (one asterisk for P < 0.05, two for P < 0.01, and three for P < 0.001)
