Table 1 Reagent sources
Reagents/resources | Source | Identifier |
Antibodies | ||
AffiniPure Goat Anti-Mouse IgG + IgM | Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA | 115–005–044 |
AffiniPure Goat Anti-Rabbit IgG (H + L) | Jackson ImmunoResearch Laboratories Inc., West Grove, PA, USA | 111–005–003 |
Anti-CD11b (Rabbit) | Abcam, Cambridge, UK | ab52478 |
Anti-CD45 (Rabbit) | Abcam, Cambridge, UK | ab10558 |
Anti-CD68 (Mouse) | Abcam, Cambridge, UK | ab201340 |
Anti-HepaCAM (Human) | R&D Systems, Minneapolis, USA | MAB4108 |
Anti-STAT3 (Rabbit) | Abcam, Cambridge, UK | ab30647 |
Anti-STAT3-P (Rabbit) | Abcam, Cambridge, UK | ab76315 |
Anti-GFAP (Rabbit, donkey) | Dako, Santa Clara, USA; Sigma, St. Louis, Missouri, USA | Z0334, G9269 |
Anti-TGFB (Rabbit) | Abcam, Cambridge, UK | ab92486 |
Anti- NeuN (Mouse) | Millipore, Massachusetts, USA | MAB377 |
Anti- IBA-1 (Rabbit) | Wako, Richmond, USA | 019–19741 |
Anti-CD11b (Rabbit) | Abcam, Cambridge, UK | Ab133357 |
Anti-α-Tubulin (Mouse) | Santa Cruz Biotechnology, Texas, USA | Sc-8035 |
Anti- Ki67 (Rabbit) | Abcam, Cambridge, UK | Ab15580 |
DAPI | Sigma, Missouri, USA | 32670 |
Goat anti-Mouse IgG Alexa Fluor 488 | Life Technologies Coorperation Eugene, USA | A11001 |
Goat anti-Rabbit IgG Alexa Fluor 568 | Life Technologies Coorperation Eugene, USA | A11011 |
Donkey anti-Goat IgG Alexa Fluor 647 | Life Technologies Coorperation Eugene, USA | A21447 |
Goat anti Rabbit IgG Alexa Fluor 488 | Life Technologies Coorperation Eugene, USA | A11008 |
Donkey anti-rabbit IgG Alexa Fluor 555 | ThermoFisher Scientific, Massachusetts, USA | |
Goat anti-Mouse IgG-HRP | Santa Cruz Biotechnology, Texas, USA | Sc-2005 |
Goat-anti-Rabbit IgG-HRP | Santa Cruz Biotechnology, Texas, USA | Sc-2004 |
Mouse-anti-Goat IgG-HRP | Santa Cruz Biotechnology,Texas, USA | Sc-2354 |
PE/CY5 conjugation kit | Abcam, Cambridge, UK | Ab 102893 |
APC/CY7 conjugation kit | Abcam, Cambridge, UK | Ab 102859 |
Ki-67 efluor 660 (SolA15) | ThermoFisher Scientific, Massachusetts, USA | 50–5698–82 |
Chemicals | ||
NeurobasalTM medium(L-Glutamine) | Gibco, Massachusetts, USA | 21103049 |
Hibernate-ATM medium | Gibco, Massachusetts, USA | A1247501 |
D+Glucose | Sigma, Missouri, USA | G8644 |
N-methyl D-Glucamine (NMDG) | Sigma, Missouri, USA | M2004 |
GlutamaxTM supplement | Gibco, Massachusetts, USA | 35050061 |
B-27TM Supplement (50×) | Gibco, Massachusetts, USA | 17504001 |
Antibiotic-Antimycotic (Anti-Anti) (100×) | Gibco, Massachusetts, USA | 15240062 |
Magnesium sulfate (MgSO4) | Sigma, Missouri, USA | M3409 |
HEPES | Sigma, Missouri, USA | H0887 |
TRIzolTM reagent | ThermoFisher Scientific, Massachusetts, USA | 15596026 |
Clodronate disodium (Dichloromethylenediphosphoric acid disodium salt) | Sigma, Missouri, USA | D4434 |
Ruxolitinib | Adipogen | AG-CRI-3624 |
Insert | ||
Millicell inserts (30 mm, 0.4 µm) | Millipore | PICMORG50 |
