Fig. 1 | Nature Communications

Fig. 1

From: Modeling microcephaly with cerebral organoids reveals a WDR62–CEP170–KIF2A pathway promoting cilium disassembly in neural progenitors

Fig. 1

WDR62 deletion results in smaller cerebral organoid sizes. a, b CRISPR/Cas9-mediated gene editing of human WDR62 locus in pluripotent stem cells (PSCs), resulting in 8 bp depletion in exon 1, and 10 or 19 bp deletion in exon 11 (a). All of them resulted in ablation of WDR62 proteins due to premature mature stop codons (b). c Confocal imaging of control and mutant PSC-derived NPCs stained with antibodies against Arl13b (red), WDR62 (green), and γ-Tubulin (blue). Scale bars: 0.5 μm. d Representative images of control and WDR62−/− cerebral organoids at week 4, 5, and 6. Scale bars: 1 mm. e Quantification of surface areas of cerebral organoids. Error bars represent SEM of three independent experiments containing numbers of organoids as indicated; ***P < 0.001 (Student’s t-test)

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