Fig. 3 | Nature Communications

Fig. 3

From: Single-site glycine-specific labeling of proteins

Fig. 3

N-terminus Gly labeling of proteins. a A user-friendly protocol delivers single-site labeling of proteins. Deconvoluted ESI-MS confirms the %conversion and rules out the possibility of side reactions (also see Supplementary Figs. 70 and 71). R indicates the fragment from reagent 2e attached to the protein. b Extension of methodology for labeling single protein (insulin 6d) in a mixture of proteins (6d–6j)

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