Fig. 5
Insulin bioactivity assay. a, Bioactivity measurement for native and modified insulin in a dose-dependent pAkt cell-based assay. The error bar represents standard error of mean (SEM). The assays were repeated at least three times (n = 3). b EC50 value determination from (a), absolute values obtained by extrapolation of graphs in a are represented by a simple bar graph. c Uptake of tagged insulin (green) and mixture of untagged and tagged insulin in cells. Chromatin (blue). Also see, Supplementary Fig. 77. d Activation of IR signaling and pAkt (red) accumulation in HEK293T cells after insulin treatment (scale bar:10 µm). Source data of Fig. 5 panel a provided as Source Data file
