Fig. 6 | Nature Communications

Fig. 6

From: Single-site glycine-specific labeling of proteins

Fig. 6

Residue-specific labeling of a protein in the cell lysate. a Selective labeling of N-Gly SUMO1 with coumarin tag in cell lysate. b 12% SDS-PAGE of cell lysate (overexpressed with SUMO1) and coumarin-tagged SUMO1 7l in cell lysate followed by coomassie staining and fluorescence imaging. MW—molecular weight, Lanes 1 and 2: cell lysate before and after reaction (Coomassie); lanes 3 and 4: cell lysate before and after reaction (fluorescence). The band at ~17 kDa (m/z 11.6 kDa) in lanes corresponds to the SUMO1. The band at ~17 kDa (m/z 11.6 kDa) shown by fluorescence imaging in lane 4 confirms the selective tagging of SUMO1 (7l). Source data of Fig. 6 panel b provided as Source Data file

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