Fig. 7
PSPH suppression blocks the expansion potential of human T-ALL cells in vivo. a Left: Schematic experimental overview of the in vivo set-up to test the effects of PSPH knockdown on leukemia engraftment and progression by tail vein injection of 1*106 scrambled control, shPSPH#1, or shPSPH#2-transduced cells 24 h after initial transduction. Right: immunoblot confirmation of PSPH knockdown in the cells that were injected in the mice. b Percentage of mCherry-expressing cells in the bone marrow of leukemic mice as determined by flow cytometry. c Spleen weights of the leukemic mice at disease end stage. Data are shown as individual data points accompanied by the median and whiskers representing data distribution. d Percentage of mCherry-expressing cells in the spleen of leukemic mice as determined by flow cytometry. All box-plots show the median and error bars define data distribution. Statistical analysis: *p-value < 0.05, **p-value < 0.01, ***p-value < 0.001. p-values were calculated using a two-tailed Student’s t-test
