Fig. 2
De novo transformation of CB cells by NOTCH1 plus LMO2-TAL1-BMI1. a Kaplan−Meier survival curves for primary recipient mice. Mice were injected with CB cells transduced with N(GFP) + LTB(Cherry) lentiviruses. Data from seven independent experimental trials are depicted with n recipient mice per trial. All leukemic animals with the exception of trial 13 (CBt13) achieved clinically morbid disease endpoints requiring euthanasia. G GFP, C Cherry. b Formalin-fixed, paraffin-embedded (FFPE) tissue histology and air-dried peripheral blood smear morphology of NLTB CB leukemias. Representative fields of tissues from multiple G+C+ leukemic animals are shown. Scale bar = 1 mm (BM upper), 20 μm (BM lower), 0.5 mm (SPL), 20 μm (PB). BM bone marrow, SPL spleen, PB peripheral blood. c BIOMED-2 TCRG clonality assay. G+C+ cells from spleens of mice with NLTB CB leukemias were FACS sorted and genomic DNA extracted for analysis. Dominant peaks indicative of clonal T-cell populations are identified in each of three NLTB CB leukemias (CBL) shown. The normal T-cell control shows a Gaussian distribution of peak sizes indicative of a polyclonal T-cell population. d, e Kaplan−Meier survival curves for secondary recipient mice transplanted with primary d NLTB CB leukemias (G+C+) or e N-only CB leukemias (G+C−). Six and five different primary leukemias, respectively, were assayed. Number of recipients for each transplantation experiment are shown in parentheses. All leukemic animals achieved clinically morbid disease endpoints requiring euthanasia
