Fig. 6
From: Small-molecule targeting of MUSASHI RNA-binding activity in acute myeloid leukemia
Ro 08–2750 demonstrates efficacy in an MLL-AF9 in vivo model. a Scheme of pharmacodynamics marker experiments with Ro short-time points performed with MLL-AF9 + secondary BM cells. 10,000 MLL-AF9 GFP + cells were transplanted and, after 3 weeks, mice were injected with DMSO or Ro (13.75 mg kg−1) and were sacrificed for analysis after 4 h and 12 h. b Surface flow analysis of c-Kit receptor in spleen cells of Ro at 4 h and 12 h versus DMSO treated mice. Results are represented as MFI of cKit-PE-Cy7 normalized to DMSO group. Each data point is an independent treated mouse. Mean ± s.e.m. is shown. c Intracellular (IC) flow analysis of c-MYC expression in spleen cells of Ro at 4 h and 12 h versus DMSO treated mice. Results are represented as MFI of c-MYC normalized to DMSO group. Each data point is an independent treated mouse. Mean ± s.e.m. is shown. d Scheme of in vivo Ro treatment in MLL-AF9 + model of myeloid leukemia. 10,000 MLL-AF9 GFP + cells were transplanted and after 3 days, mice were injected with DMSO or Ro 13.75 mg kg−1 (in DMSO) intraperitoneally (IP) at days 1, 4, 7, 10, and 13 (one day on, two days off drug). At day 19 of treatment, mice were sacrificed for organ weight and flow cytometry analysis of disease burden and MSI2 target, c-MYC. e Spleen weights at time of sacrifice. Results are represented in weight (g) and each data point represents an individual DMSO or Ro treated mouse. f White blood cell (WBC) counts (K μL−1) at time of sacrifice. Each data point represents an individually treated mouse. g Intracellular (IC) flow analysis of c-MYC expression in spleen cells of Ro versus DMSO treated mice. Results are represented as % frequency (% freq) of c-MYC + cells. Each data point is an independent treated mouse. Mean ± s.e.m. is shown. For all graphs, unpaired t-test; *p < 0.05, **p < 0.005. Source data are provided as a Source Data file
