Fig. 1

Genome and assembly features of B. lactucae. a Estimation of genome size of heterokaryotic isolate C82P24 by flow cytometry. The nuclei of B. lactucae have two peaks calibrated relative to the reference nuclei of Oryza sativa (Os, 2 C = 867 Mb). Nuclei of isolate C82P24 (P24) were estimated to be 305 Mb (P24 1, 2 C) and 599 Mb (P24 2, 4 C). Another 38 isolates all have similar sizes (Supplementary Table 1). b Extensive collinearity between B. lactucae and P. sojae displayed as a SyMap plot. c Comparison of heterozygosity in 54 isolates of 22 oomycete species (Supplementary Table 9). Dots, representative of a single isolate, are joined by bars to aid interpretations of each species. d High quality of B. lactucae assembly demonstrated by inclusion of k-mers from paired-end reads in the assembly. Colors indicate presence of k-mers in the assembly, relative to reads. Black: the distribution of k-mers present in the read set but absent in the assembly. Red: K-mers present in the read set and once in the assembly. Purple: K-mers present in the read set and twice in the assembly. The first peak depicts heterozygous k-mers and the second peak depicts homozygous k-mers. A high-quality consensus assembly will contain half the k-mers in the first peak, the other half of which should be black due to heterozygosity, and all the k-mers in the second peak should be present only once, which therefore should be red. Very few duplicated k-mers were detected in the SF5 assembly. K-mers derived from repeat sequences have higher multiplicity and are not plotted. Source data for panel c is provided in the Source Data file