Fig. 3
From: The prion-like domain of Drosophila Imp promotes axonal transport of RNP granules in vivo
Imp PLD promotes efficient localization to axons in vivo. a, b Medial lobes of adult brains expressing GFP-Imp (a) or GFP-Imp-ΔPLD (b) under the control of the 201Y-Gal4 driver. c Distributions of normalized GFP signal intensities in distal axons. d, e Magnified views of the distal region of medial γ lobes expressing GFP-Imp (d) or GFP-Imp-KH1–4DD (e), a form that cannot bind RNA and does not form granules (see Fig. 5). GFP-Imp-KH1–4DD is used to show the specificity of the granular pattern. Note that the image shown in d was not taken from the brain shown in a. Still, the approximate position of the magnified region is boxed in a. f, g Medial lobes of adult brains homozygous for the G080-GFP-Imp (f) or G080-GFP-Imp-CRISPR-ΔPLD (g) chromosomes. Note that the cell bodies of surrounding cells also expressing Imp are visible in f, g. GFP signals are shown with the Fire look-up table of ImageJ. h Distributions of normalized GFP signal intensities in distal axons. i, j Magnified views of the distal region of G080-GFP-Imp (i) or G080-GFP-Imp-CRISPR-ΔPLD (j) medial γ lobes. Note that images in i, j were not taken from the brains shown in f, g. Still, the approximate position of the magnified regions is boxed in f, g. Distributions of normalized GFP signal intensities in distal axons. k, l Medial lobes of adult brains expressing GFP-hIMP1-drosPLD (k) or GFP-hIMP1 (l) under the control of the 201Y-Gal4 driver. m Distributions of normalized GFP signal intensities in distal axons. The dashed lines in a–l delimit the distal part of MB γ axon bundles (medial lobes; see Fig. 2a). Genotypes: 201Y-Gal4/UAS-GFP-Imp (a, d); 201Y-Gal4/UAS-GFP-Imp-ΔPLD (b); 201Y-Gal4/UAS-GFP-ImpKH1–4DD (e); 201Y-Gal4/UAS-GFP-hIMP1-drosPLD (k) and 201Y-Gal4/UAS-GFP-hIMP1 (l). All box plots are represented using the min to max convention, where the middle line defines the median and the whiskers go down to the smallest value and up to the largest. Numbers of MB analyzed: UAS-GFP-Imp: 25; UAS-GFP-Imp-ΔPLD: 24; G080-GFP-Imp protein-trap: 33; G080-GFP-Imp-CRISPR−ΔPLD: 32; UAS-GFP-hIMP1: 11; UAS-GFP-hIMP1-drosPLD: 12. *** P < 0.001 (Mann–Whitney test). Scale bars in a, b, f, g, k, l: 15 μm. Scale bars in d, e, i, j: 3 μm. Source data are provided as a Source Data file
