Fig. 5
From: The prion-like domain of Drosophila Imp promotes axonal transport of RNP granules in vivo
Imp PLD is dispensable for RNP granule assembly. a Cell bodies of wild-type adult MB γ neurons stained with anti-Imp antibodies. b, d, g Cell bodies of adult MB γ neurons expressing wild-type GFP-Imp (b), GFP-Imp-ΔPLD (d) or GFP-Imp-KH1–4DD (g) under the control of the 201Y-Gal4 driver. Complete genotypes: 201Y-Gal4/UAS-GFP-Imp, 201Y-Gal4/UAS-GFP-Imp-KH1–4DD, and 201Y-Gal4/UAS-GFP-Imp-ΔPLD. c, e Cell bodies of adult MB γ neurons homozygous for the G080-GFP-Imp (c) or G080-GFP-Imp-CRISPR−ΔPLD (e) chromosomes. GFP signals are shown in white. Note that the KH1–4DD mutations induce an accumulation of Imp proteins in the nucleus, as described for the vertebrate protein68. Scale bar in a–g: 10 μm. f EMSA analysis using fluorescently-labeled profilin 3’UTR in the absence (−) or presence (+) of 800 nM recombinant GFP-Imp (left) or GFP-Imp-KH1–4DD (right)
