Fig. 6
From: The prion-like domain of Drosophila Imp promotes axonal transport of RNP granules in vivo
Imp PLD promotes Imp granule motility. a, b Single image (a) and kymograph (b) extracted from a video generated from a G080-GFP-Imp hemizygous pupal brain (24 h APF; see also Supplementary Movie 1). The bundle of MB γ axons is delimited by dotted black lines. The arrowhead points to a GFP-Imp granule. Scale bar: 10 μm. c Average number of motile anterograde, retrograde and bidirectional GFP-Imp granules per 12 min-long movie. Numbers of movies analyzed: n = 17 for G080-GFP-Imp and 16 for GFP-Imp-CRISPR-ΔPLD. Total numbers of granules analyzed: 468 for G080-GFP-Imp and 284 for G080-GFP-Imp-CRISPR-ΔPLD. d Mean anterograde and retrograde velocities. Numbers of anterograde granules analyzed: 271 (G080-GFP-Imp) and 178 (G080-GFP-Imp-CRISPR-ΔPLD); numbers of retrograde granules analyzed: 192 (G080-GFP-Imp) and 91 (G080-GFP-Imp-CRISPR-ΔPLD). Error bars in c, d represent s.e.m. *P < 0.05 (Unpaired t-test). ns stands for not significant. Source data are provided as a Source Data file
