Table 1 Data collection and refinement statistics
From: Inhibition of CRISPR-Cas9 ribonucleoprotein complex assembly by anti-CRISPR AcrIIC2
| Â | AcrIIC2 | AcrIIC2 | Se-AcrIIC2 | AcrIIC2-Nme1Cas9 | AcrIIC2-Nme1Cas9-proteolysis |
|---|---|---|---|---|---|
Data collection | |||||
PDB code | 6N05 | 6JD7 | – | 6JDJ | 6JDX |
Space group | P43212 | C2 | P6322 | P212121 | P212121 |
Cell dimensions | |||||
    a, b, c (Å) | 71.9, 71.9, 135.1 | 105.6, 73.5, 81.1 | 73.6, 73.6, 105.8 | 61.4, 77.4, 106.9 | 56.3, 77.4, 107.5 |
α, β, γ (°) | 90.0, 90.0, 90.0 | 90.0, 129.8, 90.0 | 90.0, 120.0, 90.0 | 90.0, 90.0, 90.0 | 90.0, 90.0, 90.0 |
Wavelength (Ã…) | 0.9789 | 0.97900 | 0.97918 | 0.97891 | 0.97894 |
Resolution (Å) | 49.23–2.5 (2.59–2.50) | 50.00–2.45 (2.49–2.45) | 40.72–2.54 (2.58–2.54) | 50.00–2.60 (2.64–2.60) | 50–2.28 (2.32–2.28) |
R merge | 0.0979 (2.157) | 0.074 (0.288) | 0.138 (2.423) | 0.154 (0.900) | 0.087 (1.071) |
Total reflections | 181,759 | 90,912 | 214,497 | 198,730 | 235,170 |
Unique reflections | 12,871 | 17,826 | 6026 | 16,424 | 21,775 |
I/σI | 23.8 (1.45) | 19.7 (4.6) | 21.4 (2.1) | 18.5 (2.3) | 26.6 (2.3) |
Completeness (%) | 99.9 (100) | 99.7 (99.7) | 99.9 (100) | 99.9 (99.4) | 99.1 (99.9) |
Redundancy | 14.1 (14.7) | 5.1 (5.1) | 35.6 (39.7) | 12.1 (9.4) | 10.8 (10.7) |
Refinement | |||||
Resolution (Å) | 49.23–2.5 | 40.00–2.45 |  | 38.68–2.60 | 45.01–2.28 |
No. reflections | 12,868 | 17,318 | Â | 15,017 | 17,199 |
Rwork/Rfree | 0.212/0.247 | 0.194/0.197 | Â | 0.194/0.227 | 0.208/0.222 |
No. atoms | |||||
    Protein | 1743 | 2781 |  | 2263 | 2226 |
    Ligand/ion | 0 | 5 |  | 0 | 4 |
    Water | 11 | 256 |  | 74 | 61 |
B-factors | |||||
    Protein | 89.21 | 42.06 |  | 42.93 | 36.83 |
    Ligand/ion |  | 48.31 |  |  | 20.00 |
    Water | 65.45 | 40.11 |  | 40.18 | 32.61 |
R.m.s. deviations | |||||
    Bond lengths (Å) | 0.008 | 0.020 |  | 0.005 | 0.019 |
    Bond angles (°) | 1.03 | 1.479 |  | 0.996 | 1.343 |
