Fig. 2 | Nature Communications

Fig. 2

From: The structural basis for release-factor activation during translation termination revealed by time-resolved cryogenic electron microscopy

Fig. 2

Cryo-EM structures of E.coli 70S ribosome bound with release factor 1. a Pre-accommodated ribosome complex bound with RF1 in a compact conformation. b Accommodated ribosome complex bound with RF1 in an extended conformation. Light blue: 50S large subunit; light gold: 30S small subunit; green: tripeptide; orange: P-tRNA; pink: mRNA; red: compact RF1; and dark blue: extended RF1. c, d Positions of domain III of ribosome-bound RF1 in pre-accommodated ribosome complex (c) and accommodated RF1-ribosome complex (d) relative to mRNA (pink), P-tRNA (orange) and tripeptide (green). e, f Close-up views of the upper peptide exit tunnel, showing tripeptide (green) in pre-accommodated ribosome complex (e) and accommodated ribosome complex (f)

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