Fig. 2
Inflammasome activation is compromised in septic patients with high mortality. a ELISA for IL-1β in PBMC supernatants and percentage of monocytes with intracellular ASC specks from septic patients within the first 24 h of admission to the surgical critical unit and control groups after NLRP3 inflammasome activation by LPS (1 μg/ml, 2 h) and ATP (3 mM, 30 min) treatment. There is a group of septic patients that release low or no IL-1β (blue dots). b ELISA for IL-1β and HMGB1 in PBMC supernatants and percentage of monocytes with intracellular ASC specks from control groups and septic patients treated as in a, separating septic patients into two groups: NLRP3 non-immunocompromised (gray bar) and immunocompromised (blue bar). c Percentage of mortality in NLRP3 non-immunocompromised (gray bar) and immunocompromised (blue bar) septic patients with regard to the total mortality of septic patients. d Kaplan–Meier representation of NLRP3 non-immunocompromised (gray line) and immunocompromised (blue line) septic patients’ survival. e Concentration of C-reactive protein (CRP) in plasma of NLRP3 non-immunocompromised (gray bar) and immunocompromised (blue bar) septic patients at day 1; dotted lines represent threshold concentration of CRP for healthy population. f SOFA and APACHEII indexes in NLRP3 non-immunocompromised (gray bar) and immunocompromised (blue bar) septic patients at day 1. g ΔSOFA calculated as the variation in SOFA between day 5 and day 1, gray dotted line represents no change with regard to day 1. h Number of days admitted to the Surgical Critical Unit and number of days of mechanical ventilation for NLRP3 non-immunocompromised (gray bar) and immunocompromised (blue bar) septic patients. Each dot represents an individual patient; average ± standard error is represented in panels a, b, e–h; exact n number for each panel is presented in Source Data file; *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significant difference (p > 0.05); Kruskal–Wallis test for a and b; Log-rank test for d; and Mann–Whitney test for e–h
