Fig. 1
From: The pore structure of Clostridium perfringens epsilon toxin
Cytotoxic activity of wild-type epsilon toxin (Etx) towards Super Dome cells. a The cytotoxic activity of trypsin-activated recombinant wild-type Etx towards Super Dome cells was determined by measuring the release of lactate dehydrogenase (LDH) from lysed cells. Results were normalized to the signal from cells treated with Dulbecco’s phosphate-buffered saline (DPBS) only (0% lysis) and cells treated with 0.9% (v/v) Triton X-100 (100% lysis). Results are presented as the mean of triplicate assays, each performed in triplicate (±SEM). b Purified Etx pore was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and visualized by Coomassie staining. Arrows indicate the positions of Etx pore (p) and Etx monomer (m). Source data are provided as a Source Data file
