Fig. 2
Induction of chemokine (C-X-C motif) ligand 8/macrophage inflammatory protein-2 (CXCL8/MIP-2) by lugdunin is TLR2/MyD88-dependent. a HEK-control or HEK-TLR2 cells were treated with 2 μM lugdunin for 5 h and subsequently the CXCL8 concentration in the supernatant was analyzed by LEGENDplexTM (BioLegend). Shown is one representative experiment of three independent experiments with two technical replicates ± s.e.m. b HEK-control or HEK-TLR2 cells were treated with increasing concentrations of lugdunin for 5 h and subsequently expression of CXCL8 was analyzed and normalized to actin. Shown is one representative experiment of three independent experiments with two technical replicates ± s.e.m. c Schematic overview of the mouse experiments: 6–8-week-old female C5BL/6 WT, MyD88-knockout (ko), or 5xTLR-ko mice were epicutaneously treated with 1.5 µg lugdunin or phosphate-buffered saline (PBS) as a control. After 24 h, mice were euthanized, 4 mm skin punches were taken, and further cultured in vitro for 10 h followed by cytokine analysis of the culture supernatant by LEGENDplexTM (BioLegend). d, e Shown are the mean concentrations of IL-1α (d) or MIP-2 (e) in the skin culture supernatant of two skin punches from four mice each ± s.e.m. Significant differences to control treatments were analyzed by an unpaired two-tailed t test (*P < 0.05; **P < 0.01; ***P < 0.001). f Representative MIP-2-stained paraffin-embedded mouse skin sections. Scale bar, 100 µM. Source data are provided as a Source Data file
