Fig. 2

Kinetics of protein expression and peptide presentation. a The kinetics of peptide presentation were determined by LC-MRM analysis of MHCI-bound peptides isolated from infected DC2.4 cells at 0, 0.5, 2.5, 6.5, 9.5 or 12.5 h post infection. Data are expressed as a percentage of maximum levels detected over the timecourse. Peptides whose abundance peaked at 6.5 h are shown in yellow, those that peaked at 9.5 h shown in red, and those that continued to increase at 12.5 h are shown in blue. b Relative peptide abundance (gray) is plotted alongside relative protein abundance (red) kinetics for the various IAV-derived proteins. Dashed lines indicate the immunodominant NP_366–374 and PA_224–233 peptides