Fig. 4

Reconstruction of the beetle scale sample imaged at ambient conditions. a, c shows a horizontal and vertical cut of a common SIRT reconstruction, and b, d are identical cuts through the NCT-based SIRT method. e Histograms of the reconstructed electron density for reconstructions of the ambient and cryogenically protected sample. The reconstruction quality was further quantified by Fourier ring correlation (f) showing improvement from 53- nm to 27 -nm resolution. Scale bars denote 2 µm