Fig. 2

Gastric tumor burden is reduced in mast cell–deficient gp130^FF tumor mice. a Representative whole mounts of pinned out stomachs, from 100-day-old gp130^FF; c-Kit^+/+ and mast cell-deficient gp130^FF; c-Kit^W-sh/W-sh mice. Scale bars = 1 mm. b Quantification of total tumor burden per mouse as in (a). Each symbol represents an individual mouse. One-way ANOVA F (DFn, Dfd) = 25.97 (2, 24). c Enumeration of total tumor number and tumor size distribution as in a from gp130^FF; c-Kit^+/+ (n = 14) and gp130^FF; c-Kit^W-sh/W-sh mice (n = 11). One-way ANOVA F (DFn, Dfd) = 24.59 (7, 92). d Quantification of CD31, Hypoxyprobe (hypoxia), BrdU (proliferation), or ApopTag (apoptosis) on immunostained gastric tumors sections. Number of mice (n) for CD31: FF; c-Kit^+/+ n = 7, FF; c-Kit^W-sh/W-sh n = 11 with ANOVA F (DFn, Dfd) = 7.075 (3, 34); Hypoxyprobe: FF; c-Kit⁺ n = 5, FF; c-Kit^W-sh n = 6 with t-test + Welch correction’s t (df) = 2.55 (5.256); BrdU: FF; c-Kit⁺ n = 3, FF; c-Kit^W-sh n = 4 with t-test’s t (df) = 4.905 (5); Apoptosis: FF; c-Kit⁺ n = 4, FF; c-Kit^W-sh n = 6 with t-test’s t (df) = 0.89 (8). e Quantification of total tumor burden per mouse of mast FF; MC^wt (genotypes: FF; Cpa3-Cre^neg; Mcl1^fl/fl and FF,Cpa-Cre;Macl1^+/+) and mast cell-deficient FF; MC^def (genotype: FF; Cpa3-Cre; Mcl1^fl/fl) mice. t-test’s t (df) = 6.72 (24). f Enumeration of the tumor number per mouse of FF; MC^wt (n = 17 mice) and mast cell-deficient FF; MC^def (n = 9 FF; Cpa3-Cre; Mcl1^fl/fl mice). One-way ANOVA F (DFn, Dfd) = 23.25 (7, 96). g CD31 angiogenic staining quantification of stomachs from (e, f). FF; MC^wt (n = 8 mice) and FF; MC^def (n = 5) with one-way ANOVA F (DFn, Dfd) = 6.79 (3, 22). h Quantification of total tumor burden in gp130^FF mice after 6 weeks administration of cromolyn (mast cell degranulation inhibitor) or vehicle. Each symbol represents an individual mouse and data was generated in three independent experiments. t-test’s p value is shown and t (df) = 2.313 (18). Data are represented as mean ± SEM, with p values p < 0.05, being considered significant. Source data are provided as a Source Data file. See also related Supplementary Fig 2