Fig. 3
From: IL-33-mediated mast cell activation promotes gastric cancer through macrophage mobilization
Mast cell depletion reduces macrophage infiltration in gastric tumors of gp130FF mice. a Representative images of tissue sections immunostained for macrophages (F4/80), B cells (B220) or T cells (CD3, CD8, Foxp3) in gastric tumor sections of gp130FF; c-Kit+/+ and mast cell-deficient gp130FF; c-KitW-sh/W-sh mice. Arrows indicate specific positive cell staining; scale bars = 100 µm. b Quantification of F4/80, B220, or CD3 expressing cells in tumor and tumor-associated submucosal layers of sections from (a). F4/80: n = 6 each group, one-way ANOVA F (DFn, Dfd) = 13.43 (3, 20); B220: n = 6 each group, one-way ANOVA F (DFn, Dfd) = 3.782 (3, 20); CD3: FF; c-Kit+/+ n = 6, FF; c-KitW-sh/W-sh n = 5, one-way ANOVA F (DFn, Dfd) = 11.55 (3, 18); CD8: FF, c-Kit+/+ n = 8, FF; c-KitW-sh/W-sh n = 6, t-test t (df) = 0.71 (12); Foxp3: FF, c-Kit+/+ n = 6, FF; c-KitW-sh/W-sh n = 8, t-test t (df) = 0.33 (12). Data from two independent experiments were analyzed. c F4/80+ cells frequency in stomach submucosa and tumors of FF; MCwt mice (n = 9) and mast cell-deficient FF; MCdef mice (n = 7 FF;Cpa3-Cre; Mcl1fl/fl). One-way ANOVA F (DFn, Dfd) = 10.81 (3, 28). d Quantification of F4/80+ cells in unaffected antrum (AN) or antrum tumor (AT) in submucosa or mucosa of mice of the indicated genotype. All groups n = 4. Submucosal: one-way ANOVA F (DFn, Dfd) = 10.12 (3, 12); Mucosal: one-way ANOVA F (DFn, Dfd) = 5.086 (3, 12). e Quantification of F4/80+ cells in unaffected antrum (AN) or antrum tumors (AT) in submucosa or mucosa of mice from the Tg(Tff1-CreERT2); Pik3caH1047R/+; Ptenfl/fl strain that either harbor (Cre+) or lack (Cre−) the Tff1-CreERT2 driver. Cre− AN n = 4, Cre+ AN and Cre+ AT n = 5 for both. Analyses from two independent experiments. Submucosal: one-way ANOVA F (DFn, Dfd) = 10.52 (2, 11); Mucosal: one-way ANOVA F (DFn, Dfd) = 7.485 (2, 11). Data are represented as mean ± SEM, with p values p < 0.05 considered being significant. Source data are provided as a Source Data file. See also related Supplementary Fig. 3
