Fig. 4
From: IL-33-mediated mast cell activation promotes gastric cancer through macrophage mobilization
Tumor burden in gp130FF mice diminishes upon macrophage depletion. a Representative whole mount of stomachs from 100-day-old mice of the indicated genotype. Scale bar = 1 mm. b Quantification of total tumor burden per mouse as in (a). Each symbol represents an individual mouse. Due to ill health some gp130F/F; Csf1r−/− mice had to be analyzed before the 100 day endpoint; average mouse age per groups were: gp130F/F; Csf1r+/+ 88.2 days, gp130F/F; Csf1r+/− 90.4 days, and gp130F/F; Csf1r−/− 81.6 days. One-way ANOVA F (DFn, Dfd) = 27.55 (2, 27). c Quantification of mast cell density in antral submucosa of indicated genotype was performed with gp130F/F; Csf1r+/+ n = 9 and gp130F/F; Csf1r−/− n = 5 biological samples from two independent experiments t-test t (df) = 0.262 (12). d Assessment of total tumor burden in gp130FF mice after 6 weeks administration of clodronate (50 μl of a clodrosome solution containing 5 mg/ml clodronate) or vehicle. Each symbol represents an individual mouse; data from two independent experiments (t-test t (df) = 2.622 (11)). e Quantification of F4/80, CD31, ApopTag, or toluidine blue (for detection of mast cells) stained sections of gastric tumors (T) and tumor-associated submucosa (SM) from gp130FF mice of the indicated treatment cohort. F4/80: all n = 5, one-way ANOVA F (DFn, Dfd) = 27.17 (3, 16); CD31: n = 6 (SM tissues), n = 5 (T tissues), one-way ANOVA F (DFn, Dfd) = 18.15 (3, 18); apoptotic cells: n = 4 (both groups), t-test t (df) = 0.27 (6); mast cells: n = 8 (vehicle) and n = 7 (Clodronate), t-test t (df) = 0.73 (13). Data are represented as mean ± SEM, with p values p < 0.05 considered being significant. Source data are provided as a Source Data file. See also related Supplementary Fig. 4
