Fig. 7
Acute NMDAR blockade ameliorates autistic-like features, but not learning and memory, in CDKL5-deficient mice. a Spontaneous alternation percentage is significantly reduced in saline-treated R59X mice in comparison to WT mice. However, acute memantine administration at either 2.5 or 5 mg kg−1 doses did not significantly rescue the spontaneous alternation deficit (WT-saline, n = 9; R59X-saline, n = 11; WT-memantine (5 mg kg−1), n = 6; R59X-memantine (5 mg kg−1), n = 6; WT-memantine (2.5 mg kg−1), n = 6; R59X-memantine (2.5 mg kg−1), n = 6; Kruskal–Wallis test with Dunn’s multiple comparisons test). b Total grooming in a home-cage like environment is significantly increased in saline-treated R59X mice in comparison to WT, and memantine at 5 mg kg−1 significantly ameliorated the increased grooming phenotype in R59X mice (n = 8 for all groups; one-way ANOVA with Holm-Sidak’s multiple comparisons test). c The duration of the longest grooming bout is significantly increased in saline-treated R59X mice in comparison to WT, and memantine also ameliorated this aspect of repetitive behavior (n = 8 for all groups; one-way ANOVA with Holm-Sidak’s multiple comparisons test). d On the dyadic social assay, saline-treated R59X mice spend significantly less time initiating social interaction with a novel stimulus mouse in comparison to WT. Memantine at 5 mg kg−1 resulted in a significant increase in time spent in social interaction (n = 7 for all groups; one-way ANOVA with Holm-Sidak’s multiple comparisons test). e For co-tested pairs of R59X and WT mice, the ratio of time spent in direct interaction was significantly reduced in saline-treated mice. Memantine normalized this ratio, indicating a differential effect of this drug on social interaction in WT and R59X mice (n = 7 for all groups; unpaired t-test). *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. Source data are provided as a Source Data fil
