Fig. 6
pAB5 modulates chromosomally-encoded proteins. Quantitative proteome analysis of the effect of pAB5 on the secretome (a), the whole proteome with shaking (b), and whole proteome under static conditions (c). Manhattan plots demonstrating the significance of protein alteration, –log10(p-value), vs position in the genome are shown. The direction of the protein alteration is colored coded according the provided heat map. Selected proteins and accession numbers are shown. Full data sets are shown in Supplementary Fig. 7. Pink shadow highlights the genes encoding for CUP1 pili, yellow shadow highlights the genes encoding for CUP2 pili, orange shadow highlights the genes of PNAG biosynthetic pathway and green shadow highlights the genes encoding for the T6SS. Four biological replicates of each condition were analyzed. The default Maxquant FDR setting of 1% FDR at the protein and peptide levels were used
