Fig. 3
From: Mechanical impact of epithelial−mesenchymal transition on epithelial morphogenesis in Drosophila
Cell delamination starts during mesoderm invagination. a Image extracted from a movie showing ventral and sagittal views of a PH-mCherry embryo during mesoderm invagination. Scale bar: 10 µm. b Time-lapse images of PH-mCherry embryo (ventral view, see scheme) showing strong apical reduction, then apical surface disappearance (yellow arrows) of cells colored in red during mesoderm invagination. Scale bar: 10 µm. c Transversal views of PH-mCherry fixed embryos at early (top) or late (bottom) stages of invagination stained with anti-Snail antibody (n = 17). Note the basal relocation of some nuclei within the mesoderm domain (Snail-positive cells, in green) at early stage and the presence of cells at different stage of delamination (outline in dashed yellow) at later stage: arrowheads point at protruding (white arrowheads) and extruded Snail-expressing cells (yellow arrowheads). Note that Snail expression is reduced in extruded cells. Scale bar: 10 µm. d Transversal view of PH-mCherry fixed embryos at late stages of invagination stained with anti-DE-cadherin antibody (n = 11). Note the presence of protruding (white arrowheads) and extruded (yellow arrowheads) cells (outline in dashed yellow). DE-cadherin is not detected in extruded cells. Scale bar: 10 µm. An anterior, P posterior, V ventral, D dorsal, Ap apical, B basal
