Fig. 6
From: Poly(ADP-ribose) polymerase-1 antagonizes DNA resection at double-strand breaks
Poly(ADP-ribose) polymerase-1 (PARP-1) knockdown or pharmacological inhibitor affects Ku80 recruitment on double-strand breaks. a U2OS cells stably expressing the Ku80-GFP were mock treated, treated with BMN 673, or silenced with PARP-1 siRNA and subjected to laser microirradiation-track studies. b Quantification of the relative fluorescence intensity of Ku80-GFP over time. Error bars represent mean with s.e.m. c Recruitment of Ku80-GFP in PARP-1-deficient CRISPR 293T cells is severely affected. d Quantification of the relative fluorescence intensity of Ku80-GFP over time. Error bars represent mean with s.e.m. e AsiS1-ER-U2OS reporter cells were cultured and treated with 4-hydroxytamoxifen (300 nM) for 1 h. Soluble chromatin fractions were prepared and subjected to immunoprecipitation against IgG control and Ku80-specific antibody. Immunoprecipitated chromatins fractions were analyzed by quantitative polymerase chain reaction using specific primer pairs: chr1_89231183. Error bars represent mean ± s.e.m. ****p ≤ 0.0001 (Mann–Whitney U test). Source data are provided as a source data file
