Fig. 7
From: Poly(ADP-ribose) polymerase-1 antagonizes DNA resection at double-strand breaks
Single-molecule analysis of resection tracks (SMART) analysis and effect of poly(ADP-ribose) polymerase-1 (PARP-1) on homologous recombination. a SMART of PARP-1-deficient mouse embryonic fibroblasts (MEFs). MEFs were treated with 10 Gy irradiation (IR). Error bars represent mean ± s.e.m. b Treatment of HeLa cells with BMN 673 results in IR sensitivity. Error bars represent mean with s.e.m. c SMART of BRCA2-proficient (DLD1 BRCA2 (+/−)) and BRCA2-deficient cells (DLD1 BRCA2 (−/−)) either mock treated of treated with BMN 673 (5 μM) were irradiated (10 Gy). Error bars represent Mean with s.e.m. d Attenuation of PARP-1 increases RAD51 foci in cellulo. Error bars represent mean ± s.e.m. e Homologous recombination is increased in PARP-1 knockdown cells. Error bar represents mean ± s.d. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 (Mann–Whitney U test). Source data are provided as a source data file
