Table 1 Results of CRISPR/Cas9-mediated targeting in C57BL/6J mice
Gene | Group | Embryos transferred (recipients) (n) | Newborns (n) | Mutant mice (n) | NHEJ efficiency (%) | Mice carrying frameshift mutations (n) | Mice carrying a clear frameshift mutation (n) | Surviving adult mutant mice (n) |
|---|---|---|---|---|---|---|---|---|
Virma | sgRNA1 zygote | 216 (10) | 0 | – | – | – | – | – |
sgRNA1 two-cell embryo | 70 (2) | 10 | 1 | 10 | 1 | 1 | 1 | |
sgRNA2 zygote | 160 (8) | 0 | – | – | – | – | ||
sgRNA2 two-cell embryo | 110 (3) | 20 | 2 | 10 | 2 | 2 | 2 | |
sgRNA3 zygote | 98 (4) | 0 | – | – | – | – | – | |
Dpm1 | sgRNA two-cell embryo | 128 (4) | 25 | 7 | 28 | 7 | 7 | 6 |
Slc17a5 | sgRNA zygote | 30 (1) | 5 | 5 | 100 | 4 | 4 | 0 |
sgRNA two-cell embryo | 51 (2) | 18 | 10 | 55.6 | 7 | 4 | 7 | |
Ctla-4 | sgRNA two-cell embryo | 41 (2) | 12 | 10 | 83.3 | 9 | 3 | 6 |
