Fig. 6
From: Actomyosin-driven force patterning controls endocytosis at the immune synapse
Characterization of actin patches. a Protrusions associated with bead movement (red) on antigen-coated gel pieces presented laterally to B cell (green), 3D reconstruction. b Electron microscopy image showing actin-rich protrusions (marked by gold beads) through PAA gel (scale bar 0.2 µm). c Immunostaining of actin (cyan) and phospho-cortactin (magenta) and zoomed orthogonal projections on different planes (scale bar 5 µm, zoom 0.5 µm); images show colocalization (quantify on the right as ratio of magenta signal in the cyan region, control is the enrichment of the signal in random position, Median ± IQR, 26 cells, two independent experiments, two mice, Mann–Whitney test). d Immunostaining of actin (cyan) and clathrin (magenta) and zoomed orthogonal projections on different planes (scale bar 5 µm, zoom 0.5 µm); images show partial colocalization (not quantified, scale bar 5 µm, zoom 0.5 µm). Source data are provided as a Source Data file
