Fig. 7
From: Actomyosin-driven force patterning controls endocytosis at the immune synapse
Actin patches correlate with antigen Internalization. a Average distribution of actin patches (obtained by tracking and convoluting the results with a Gaussian kernel): internal circle (2/3 cell diameter) corresponds to the central portion of the cell (scale bar represents the integrated density as number/cell/5 min). The distribution in HEL-coated gel is very different compared with the BSA coated gel in b number of beads (Mann–Withney test, Median ± IQR) and c radial distribution (BSA: 25 cells, HEL: 34 cells, three different mice). d Effective diffusion coefficient of actin patches (violin plot with Median ± IQR, n = 301 trajectories (34 cells) for HEL and n = 84 trajectories (25 cells) for BSA, Mann–Whitney test). e Time lapse of the extraction of fluorescent HEL (below: percentage of the maximum, scale bar 3 µm). f Strain energy and antigen gathering (Mean ± SEM, n = 15, five independent experiments); signals are normalized to the maximum to highlight similarity in the kinetics. g Example of colocalization of the actin patches (lifeact-GFP) and fluorescent HEL (AF555), bottom linescan on the colocalization panel (scale bar 3 µm). h Zoom on an actin (and HEL) patch showing the concomitant arrival of the two signals. i Quantification of the signal in g measured over a square of 1 µm × 1 µm: both signals appear simultaneously (mean ± SEM, n = 21, from six cells). Source data are provided as a Source Data file
