Fig. 1

Loss of TCER-1 enhances resistance against multiple biotic and abiotic stressors. Survival of L4 stage, wild-type C. elegans (WT, black), tcer-1 (blue), glp-1 (green) and tcer-1;glp-1 (orange) mutants exposed to different stressors. a–c Pathogen exposure. a P. aeruginosa PA14: WT (m = 59.35 ± 1.3, n = 89/106), tcer-1 (m = 98.6 ± 3.5, n = 83/109, P vs. WT <0.001), glp-1 (m = 72.86 ± 2.1, n = 102/109, P vs. WT <0.0001) and tcer-1;glp-1 (m = 114.18 ± 3.9, n = 81/106, P vs. glp-1 <0.001). b P. aeruginosa PA01: WT (m = 126.43 ± 2.4, n = 118/142), tcer-1 (m = 139.98 ± 2.7, n = 143/173, P vs. WT <0.001). c S. aureus: WT (m = 94.67 ± 1.5, n = 97/111), tcer-1 (m = 109.27 ± 1.5, n = 145/169, P vs. WT <0.001). d Heat stress: survival of day 2 adults 12 h after exposure to 35 °C for 6 h. n = 50−100 animals per strain per each of six biological replicates analyzed using unpaired two-tailed t test. e Oxidative stress: survival of L4 animals in 7 mM t-BOOH. WT (m = 17.32 ± 1.1, n = 57/98), tcer-1 (m = 25.65 ± 1.7, n = 33/100, P vs. WT <0.0001), glp-1 (m = 27.88 ± 1.4, n = 79/100, P vs. WT <0.0001) and tcer-1;glp-1 (m = 56.31 ± 5.1, n = 29/100, P vs. glp-1 <0.0001). f DNA damage: viability of eggs laid by day 1 hermaphrodites 24 h after exposure to 50 Gy γ-irradiation, n = 9−20. P 0.003 in unpaired t test. In (a–c) and e, survival data analyzed using Kaplan−Meier test, shown as mean lifespan in hours (m) ± standard error of the mean (SEM). ‘n’ refers to number of animals analyzed/total number in experiment (see Methods for details). P values adjusted for multiplicity where applicable. Asterisks indicate statistical significance <0.01 (**), <0.001 (***) and <0.0001 (****) and their color denotes the strain of comparison. Data from additional trials presented in Supplementary Tables 1A, B (panel a), 2A, B (b, c), 3A (e) and Supplementary Fig. 1d (f)