Table 1 Cryo-EM data collection, refinement, and validation statistics
(EMDB-4508) (PDB 6QCM) | (EMDB-4509) | |
|---|---|---|
Data collection and processing | ||
Magnification | 130,000 | 130,000 |
Voltage (kV) | 300 | 300 |
Total electron dose (e−/Å2) | 45 | 45 |
Defocus range (μm) | 1.4 to −4.1 μm | 1.4 to −4.1 μm |
Pixel size (Å) | 1.06 | 1.06 |
Symmetry imposed | C1 | I1 |
Initial particle images (no.) | 249,957 | 249,957 |
Final particle images (no.) | 78,084 | 78,084 |
Map resolution (masked Å) Map resolution (unmasked Å) FSC threshold | 4.48 6.32 0.143 | 3.38 3.60 0.143 |
Refinement | ||
Model resolution (Å) FSC threshold | 4.22 0.5 | |
Model resolution range (Å) | 3.0–5.8 | |
Map sharpening B factor (Å2) Model composition | ||
Non-hydrogen atoms Protein residues | 52,618 7000 | |
B factors (Å2) | ||
Protein | 62.10 | |
R.m.s. deviations | ||
Bond lengths (Å) Bond angles (°) | 0.006 1.17 | |
Validation | ||
MolProbity score Clashscore Poor rotamers (%) | 2.26 7.42 1.16 | |
Ramachandran plot | ||
Favored (%) Allowed (%) Disallowed (%) | 84.49 15.17 0.34 | |
