Fig. 2
From: NLRC5 inhibits neointima formation following vascular injury and directly interacts with PPARγ
NLRC5 attenuates neointimal formation in vivo. a Schematic diagram of generating Nlrc5 knockout construct. Recombination of the Nlrc5−/− allele is examined by PCR in the Nlrc5+/+, Nlrc5+/−, and Nlrc5−/− mice. b Representative images of hematoxylin/eosin-stained Nlrc5−/− and Nlrc5+/+ mice carotid arteries at 3 weeks after carotid ligation. Scale bar: 100 μm (upper) and 50 μm (lower). c, d Quantification of the intima area and intima/media ratio in the histological sections (n = 7 per group). e, f Immunohistochemistry staining (red arrow) and quantitative analysis of PCNA-positive cells in the carotids (n = 8 per group). Five fields per section from each sample are analyzed. Scale bar: 100 and 20 μm. g Western blotting of Flag tag and β-actin in the ligated Ad-Control- and Ad-Nlrc5-transducted carotids at 5–10 days following carotid ligation. h Representative images of hematoxylin/eosin-stained carotid arteries transducted with Ad-Control or Ad-Nlrc5 at 3 weeks after carotid ligation. Scale bar: 50 μm (upper) and 20 μm (lower). i, j Quantification of the intima area and intima/media ratio in the histological sections (n = 6 per group). k, l Immunohistochemistry staining (red arrow) and quantitative analysis of PCNA-positive cells in the carotids transducted with Ad-Control or Ad-Nlrc5 (n = 6 per group). Five fields per section from each sample are analyzed. Scale bar: 20 μm. Data are presented as mean ± SD. Two-tailed Student’s t-test is used to compare two groups (c, d, f, i, j, and l). *P < 0.05. Original magnification, × 200 (b and h) and × 400 (b, e, h, and k). Source data are provided as a Source Data file
