Fig. 1

dsRNA and RA cellular response signatures overlap. a A 1550 nm nonablative fractional laser treatment improves photoaging. b, c After 7 days of laser treatment, among the most induced transcripts are the OAS family (OAS1, 2, 3, and L) and enzymes in RA pathway (RDH16, RAET1E, DHRS9) (b); unsupervised upstream regulator analysis confirms strong signatures for dsRNA and Retinoic Acid (RA) induced signaling (c). d, e Venn diagrams show high overlap in comparisons of top ranked 100 upregulated and downregulated transcripts (d; from 49,395) or proteins (e; from 3814) between RA- and dsRNA (Poly (I:C); 30 µg per ml)-treated foreskin keratinocytes. f Gene ontology analysis of overlap proteins in e. g Keratinocyte genes upregulated with dsRNA depend on the dsRNA receptor TLR3, but also RARG. Depicted are mRNAs, and western protein analysis of KRT19. (n = 3–4 independent experiments, ***P < 0.001, *P < 0.05, one-way ANOVA). Data are means ± SEM