Fig. 1
From: The deubiquitylase OTUD3 stabilizes GRP78 and promotes lung tumorigenesis
Comparison of tumorigenesis in OTUD3 WT and KO mice. a Representative bright-field imaging of mammary adenocarcinoma from WT, OTUD3 KO, PyMT/WT, and PyMT/OTUD3 KO littermates and arrows indicate surface tumors. b Mammary adenocarcinoma incidence in PyMT/WT (n = 8) and PyMT/OTUD3 KO (n = 8) littermates depicted as the percentage of tumor-free mice. Mice were considered to be tumor free until a palpable mass (>4.0 mm) persisted for longer than 4 days. Log-rank test. c Representative whole-body posterior and left lateral images of PyMT/WT and PyMT/OTUD3 KO littermates with breast tumors obtained from single-photon emission computed tomography (SPECT)/CT imaging at 0.5 h after 99mTc-HHK injection. d Total tumor volume of PyMT/WT (n = 6) and PyMT/OTUD3 mice (n = 6) were analyzed once a week after tumor formation. Two-way ANOVA test. e, f The tumor at the fifth week of indicated mice were obtained and calculated (PyMT/WT, n = 6; PyMT/OTUD3, n = 6). Mann–Whitney test. g Immunohistochemistry analysis protein levels of PTEN, p-AKT(S473), Ki-67, CD31, and cleaved Caspase-3 (scale bar, 100 μm) in breast tumors of PyMT/WT and PyMT/OTUD3 KO littermates, respectively. h Kaplan–Meier plot showing overall survival of mice with the indicated genotypes infected with AdCre (KrasG12D/WT/WT, n = 16; KrasG12D/WT/OTUD3 KO, n = 10), log-rank test. i, l Micro-CT images in indicated planes from OTUD3 WT or KO mice (i) and OTUD3 WT or TG mice (l) indicated weeks post-infection (1 × 1010pfu AAV-GFP-Cre). j, m Three-dimensional rendering of micro-CT data with lungs in gray, lost part represented tumor. k, n Representative images of lung lesions (k, n, left, scale bar, 0.5 cm) and H&E staining (k, n, right, scale bar, 0.5 cm) from the indicated experimental group and arrows indicate surface tumors. For the box and whisker graphs in f, the box extends from the twenty-fifth to the seventy-fifth percentile, the line inside the box is the median and the whiskers are the fifth and ninety-fifth percentiles. Statistics source data can be found in Supplementary Data 1
