Fig. 5
From: Mesophyll porosity is modulated by the presence of functional stomata
Mesophyll porosity is modulated by gas exchange through stomatal pores. a–d 3D microCT renderings of tissue blocks (resolution = 2.75 μm; samples = 1.1 mm2) and (e–h) exemplar SEM images of stomata from leaves of Arabidopsis EPF2-OE, Col-0, focl1-1 and epf1epf2 lines (scale bars = 10 μm). Means and standard deviation are shown for (i) stomatal density (ANOVA, F(3,11) = 19.17, p < 0.0001), (j) palisade mesophyll porosity (ANOVA, F(3,20) = 6.329, p = 0.0034) and (k) stomatal conductance, gs (ANOVA, F(3,20) = 26.22, p < 0.0001) for the Arabidopsis lines in a–d, with n = 6 except for stomatal density where n = 4 (focl-1), n = 2 (EPF2-OE) and n = 3 (epf1epf2). Col-0 data are as in ref. 13. Lines indicated with the same letter cannot be distinguished from each other at the p < 0.05 confidence limit (posthoc Tukey). l Palisade mesophyll porosity is plotted against stomatal conductance, gs, for individual leaf samples from the four Arabidopsis lines, as indicated. Linear regression results are presented
