Fig. 1
From: A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo
Design strategy and initial screening of frankenbodies. a A cartoon schematic showing how to design a chimeric anti-HA scFv using 12CA5-scFv CDRs and stable scFv scaffolds. b A cartoon showing how to screen the five chimeric anti-HA scFvs in living U2OS cells. c Initial screening results showing the respective localization of the five chimeric anti-HA scFvs in living U2OS cells co-expressing HA-tagged histone H2B (chimeric anti-HA scFv, green; 4 × HA-mCh-H2B, magenta). From left to right, n = 17, 33, 27, 31, 25 cells. d Control results showing the respective localization of \(\chi _{15{\mathrm{{F}}}11}^{{\mathrm{{HA}}}}\) and \(\chi _{2{\mathrm{{E}}}2}^{{\mathrm{{HA}}}}\) in living cells lacking HA-tagged histone H2B (chimeric anti-HA scFv, green; mCh-H2B, magenta). From left to right, n = 21, 15 cells. e Nuclear to cytoplasmic fluorescent intensity ratio (Nuc/Cyt) plot of each chimeric anti-HA scFv for all cells imaged as in c and d. Student’s t-test. ****p < 0.0001. All images are representative cell images from one independent experiment. Scale bars: 10 µm. Source data are provided as a Source Data file. For the box and whisker plots, median is shown by a white line, the box indicates 25–75% range, and whiskers indicate 5–95% range
