Fig. 3
From: A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo
Using purified recombinant frankenbody in vitro. Immunostaining in fixed U2OS cells with purified frankenbody (FB-GFP; green) of an HA-tagged (a) nuclear protein, histone H2B (4 × HA-mCh-H2B; magenta; a representative cell image of n = 27 cells in one independent experiment) and (b) cytoplasmic protein, β-actin (4 × HA-mCh-β-actin; magenta; a representative cell image of n = 10 cells in one independent experiment). c Western blot of HA-tagged H2B and β-actin. Left: purified FB-GFP (1:2000 dilution, no secondary antibody) detected directly using GFP fluorescence; Right: parental anti-HA antibody 12CA5 (1:2000 dilution) detected with secondary anti-mouse antibody/Alexa488 (1:5000 dilution). Scale bars, 10 µm
