Fig. 4
From: A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo
HA frankenbody binds the HA epitope for minutes in cells. a A representative FRAP experiment (yellow circle indicates bleach spot) showing fluorescence recovery in cells expressing frankenbody (FB-GFP; green) and target 4 × HA-mCh-H2B (magenta). b Quantification of FRAP data in a representative cell, along with a fitted curve. c A representative FRAP experiment (yellow circle indicates bleach spot) in cells expressing FB-GFP only (i.e. cells lacking HA-tags) is complete in less than 10 s. d Half recovery time plot of FRAP experiments (FB-GFP and 4 × HA-mCh-H2B, n = 12 cells in 2 independent experiments, as in b) and controls (FB-GFP only, n = 8 cells in one independent experiment, as in c). Fits from 12 cells reveal the FRAP mean half recovery time, thalf, is 141 ± 7 s (cell-to-cell SEM). Student’s t-test. ****p < 0.0001. Scale bars, 10 µm. Source data are provided as a Source Data file. For the box and whisker plots, median is shown by a white line, the box indicates 25–75% range, and whiskers indicate 5–95% range
