Fig. 9
From: A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo
Monitoring HA-tagged proteins in zebrafish embryos. a Max-projection images from a zebrafish embryo with frankenbody (FB-GFP) and 4 × HA-mCh-H2B. Cy5-Fab labels nuclear histone acetylation as a positive control. b A nuclei (dash circle in a) and its progeny tracked in development. The nuclear to cytoplasmic ratio (Nuc/Cyt) from FB-GFP (green circles), HA-H2B-mCh (magenta squares), and Fab (gray diamonds) in the parental nuclei (dotted line). c Cell count (top), average nuclear area (units of pixel2 with one pixel = 662 nm, middle) and Nuc/Cyt ratios for all tracked nuclei. See also Supplementary Figs. 7, 8 and Supplementary Movies 10, 11. Two embryos in two independent experiments. Error bars, SEM. Scale bar, 50 mm. Source data are provided as a Source Data file
